Why SH-SY5Y cells should be differentiated?
It is therefore critical that when designing an experiment focused on testing neurons in vitro, SH-SY5Y cells should be differentiated in order to obtain the most accurate results for translation and comparison to in vivo models.
What is SH-SY5Y cells?
SH-SY5Y is a thrice-subcloned cell line derived from the SK-N-SH neuroblastoma cell line. It serves as a model for neurodegenerative disorders since the cells can be converted to various types of functional neurons by the addition of specific compounds.
What is neuroblastoma cell line?
Abstract. Neuroblastoma (NB) cell lines are transformed, neural crest derived cells, capable of unlimited proliferation in vitro. These cell lines retain the ability of differentiation into neuronal cell types on treatment with various agents.
Which medium and ingredients provide better morphological differentiation of SH-SY5Y cells?
Our results showed that RA-Mix-BDNF medium has the best differentiation capacity on the SH-SY5Y cells and we suggest using the medium for neuroscience studies.
What are BV 2 cells?
BV2 cells are a well-characterized, extensively employed model system for microglia. Studies have demonstrated that BV2 cells are a valid substitute for primary microglia in many experimental settings, including complex cell-cell interaction studies (Henn et al., 2009).
Are neuroblastoma cells neurons?
Neuroblastomas and neuroblastoma cell lines contain cells that resemble immature sympathetic neurons (sometimes called N-type cells) and Schwann cells (S-type cells).
Where is HEK293 from?
HEK293 is a cell line derived from human embryonic kidney cells grown in tissue culture. They are also known, more informally, as HEK cells. This particular line was initiated by the transformation and culturing of normal HEK cells with sheared adenovirus 5 DNA.
Where is microglia located?
the brain
Microglia are a type of neuroglia (glial cell) located throughout the brain and spinal cord. Microglia account for 10–15% of all cells found within the brain. As the resident macrophage cells, they act as the first and main form of active immune defense in the central nervous system (CNS).
Can neurons be cancerous?
Neurons are special cells that link together to transmit information and respond to the world around us. Also, neurons do not undergo mitosis and so rarely become cancerous as they do not duplicate themselves.