Which media used for Azotobacter?
Species of Clostridium capable of fixing nitrogen can be isolated from the soil by using the same media as used for Azotobacter, that is nitrogen-free mannitol broth, and nitrogen-free mannitol agar.
Which Agar is used for the cultivation of Azotobacter?
Azotobacter Agar (Glucose) is used for isolation and cultivation of glucose positive Azotobacter species from soil (2). It is also useful for maintenance of Azotobacter species by adding extra 1% glucose to the medium as specified by the American Type Culture Collection (3).
Which selective media is used for Azotobacter isolation?
Azotobacter Agar (Mannitol) is used for isolation and cultivation of mannitol positive Azotobacter species from soil (4). It is also useful for maintenance of Azotobacter species by adding extra 1% Mannitol to the medium as specified by the American Type Culture Collection (1).
Can Azotobacter grow on nutrient agar?
Isolation of Azotobacter from Mixed Culture:- Colonies that are identified to be Azotobacter, after Gram’s staining, are sub-cultured on Nutrient agar plates.
Is Azotobacter media selective?
Azotobacter media is selective because it contains no fixed nitrogen source.
What is Yema medium?
Yeast extract mannitol agar (YEMA) is the most commonly used medium for the culture of rhizobia, and is made from either yeast water preparations, or commercial yeast extract powders and pastes.
Why is Ashby’s mannitol agar used for Azotobacter?
Ashby’s Mannitol Agar is used for cultivation of Azotobacter species by using mannitol as carbon source. Azotobacter is a genus of free-living diazotrophic bacteria which have the highest metabolic rate. Azotobacters are chemoorganotrophic, using sugars, alcohols and salts of organic acids for growth.
How do you make a medium Ashby?
Preparation Instructions Suspend 40.7 grams of Ashby’s Mannitol Agar in 1000 mls of distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes.
How do you isolate Azotobacter from soil samples?
Procedure:
- Pour Ashby’s medium into sterile Petri plates and allow them to solidify.
- Sieve the soil through 2 mm sieve, weigh two 10 g samples, keep one sample in an oven over night at 150°C.
- Add the other 10 g soil sample into the 90 ml water blank, shake for 20-25 minutes on the magnetic shaker.
Can Azotobacter grow on tryptone soy agar?
general purpose nutrient media, Difco Tryptic Soy Agar (TSA) , gives rise to somewhat smaller, more discrete colonies than does Burk’s agar and the plates are much easier to count. This medium can be used satisfactorily to grow many strains of Azotobacter including A.
What is YEM Agar?
YEM Agar is widely used for the cultivation of Agrobacterium species and other soil microorganisms. Agrobacterium is a genus of Gram negative bacteria. The Agrobacterium genus is quite heterogenous Agrobacterium is well known for its ability to transfer DNA between itself and plants.
What is yeast mannitol agar?
Yeast Mannitol. Agar is used for the cultivation of symbiotic nitrogen fixing organisms viz. Rhizobium species (1) Yeast extract serves as a good source of readily available amino acids, vitamin B complex and accessory growth factors for Rhizobia.