What is the restriction site for BamHI?
Restriction Enzyme Cut Site: G/GATCC.
How many restriction sites are there for BamHI?
Two-metal ion mechanism is the use of two metal ions to catalyze the cleavage reaction of restriction enzyme. BamHI has three critical active site residues that are important for metal catalyst.
Where does the restriction enzyme HindIII cut?
HindIII (pronounced “Hin D Three”) is a type II site-specific deoxyribonuclease restriction enzyme isolated from Haemophilus influenzae that cleaves the DNA palindromic sequence AAGCTT in the presence of the cofactor Mg2+ via hydrolysis.
What is the source of BamHI restriction enzyme?
BamHI is a type II restriction enzyme derived from Bacillus amyloliquefaciens. Like all Type II restriction endonucleases, it is a dimer and the recognition site is palindromic and 6 bases in length.
Does BamHI produce blunt ends?
Cleavage with two restriction endonucleases that produce blunt ends. Cleavage with two restriction endonucleases that produce compatible overhangs….Compatible Cohesive Ends and Generation of New Restriction Sites.
| Enzyme | Ligated To | Recleaved By |
|---|---|---|
| BamHI * (G/GATCC) | BstYI (G/GATCC) | AlwI, BamHI, BstYI, DpnII, NlaIV |
| BanI | ||
| (G/GTACC) | Acc65I | Acc65I, BanI, KpnI, NlaIV, RsaI |
What is the sequence of the sticky end that results when DNA is cut with BamHI with HindIII?
HindIII – recognises the sequence 5’AAGCTT’3 – sticky ends. PstI – recognises the sequence 5’CTGCAG’3 – sticky ends. Sau3A – recognises the sequence 5’GATC’3 (produces the same sticky ends as BamHI upon cutting) HaeIII – recognises the sequence 5’GGCC’3 – blunt ends.
How do EcoRI and BamHI differ?
The BamHI recognition sequence differs by only one base pair in each half site from the EcoRI sequence 5′-GAATTC-3′. Both enzymes cleave the DNA at iden- tical positions, following the 5’G on each strand. A subunit of BamHI consists of 213 amino acid residues [11].
What is the recognition site of HindIII?
Thermo Scientific HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in R buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.
Where is HindIII located?
Introduction. Endonuclease HindIII is a type II restriction enzyme which recognizes and cleaves the palindromic sequence AAGCTT in the presence of Mg2+. The gene encoding HindIII endonuclease (R) is located upstream of its cognate methyltransferase gene (M).
How many fragments are produced by BamHI?
Under ideal conditions there would be 6 fragments from Enzymes A and B, and 8 fragments from Enzyme C. GGATCC is the recognition site for BamHI and is found in λ DNA at 5 locations.
What is the sequence of the sticky end produced by BamHI?
BamHI binds at the recognition sequence 5′-GGATCC-3′ , and cleaves these sequences just after the 5′-guanine on each strand. This cleavage results in “sticky ends” which are 4 b.p. long.
Which restriction enzyme gives blunt ends?
Eco RV
So, the correct answer is ‘Eco RV’.