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What is Sedi stain?

What is Sedi stain?

Highly selective formula stains blood cells, casts, and other formed elements in urine sediment in a distinctive fashion that permits rapid and accurate identification. Sedi-Stain is used directly from the dropper bottle and will remain stable indefinitely.

How can you tell the difference between RBC and WBC in urine?

Identification. In regular unstained urine sediments, WBC are small cells, usually 1.5 – 2 x larger than a RBC (see image below), are regularly round, colorless and have a slightly grainy appearance. Like erythrocytes, WBC may lyse in very dilute or highly alkaline urine.

What is the principle of urinalysis?

PRINCIPLE: Routine urinalysis consists of color, clarity specific gravity and a chemical examination including pH, albumin, glucose, ketones, bile, leukocyte esterase, nitrite, and blood. Microscopic examination, if indicated, includes cell identification, casts, crystals, bacteria, and miscellaneous.

Which is the correct way to analyze urine microscopically?

Microscopic urinalysis is often done as part of an overall urinalysis. After a urine (pee) sample is collected, it’s put into a centrifuge — a special machine that separates the liquid in the urine from any solid components that may be present, such as blood cells, mineral crystals, or microorganisms.

How do you use urine sediment stains?

Add one drop of the stain to the tube, using a pipette. Re-suspend the sediment in the supernatant by gently swirling or “finger-flicking” the tube. Use a pipette to transfer one drop of the reconstituted sediment to a microscope slide. Place a coverslip over the sample.

What is the Colour of RBC?

A protein called hemoglobin inside red blood cells is the transport molecule that allows red blood cells to carry oxygen throughout the body. It also has a characteristic red pigment, giving blood its red color.

What is the Colour of WBC?

Contrary to their name, white blood cells are colorless but can appear as a very light purple to pink color when examined under a microscope and colored with dye. These extremely tiny cells have a round shape with a distinct center membrane (nucleus).

What are the 4 components of a urinalysis?

A complete urinalysis consists of three components or examinations: physical, chemical, and microscopical. Physical examination describes the volume, color, clarity, odor, and specific gravity.

What are the five components of a urinalysis?

The squares on the dipstick represent the following components in the urine:

  • Specific gravity (concentration of urine)
  • Acidity of the urine (pH levels)
  • Protein in the urine (proteinuria), mainly albumin.
  • Glucose (sugar) in the urine (glycosuria)
  • Ketones in the urine (ketonuria), products of fat metabolism.

What is the aim of urine microscopy?

What is this test? This test looks at a sample of your urine under a microscope. It can see cells from your urinary tract, blood cells, crystals, bacteria, parasites, and cells from tumors. This test is often used to confirm the findings of other tests or add information to a diagnosis.

How should the supernatant and sediment be separated?

Decant the supernatant off the sediment, discarding it in a sink. Leave approximately 0.5-1ml of supernatant in the tube. Close the lid and resuspend the sample by gently flicking the tube with your finger or mixing the content with a pipette.