What is RNAlater used for?
Invitrogen RNAlater Stabilization Solution is an aqueous, nontoxic tissue RNA stabilization and storage reagent that rapidly permeates tissues to stabilize and protect cellular RNA. RNAlater solution minimizes the need to immediately process tissue samples or to freeze samples in liquid nitrogen for later processing.
Does RNAlater fix cells?
No, the tissue is not fixed, and RNAlater does not really fix it, it’s a RNA protection agent.
What is RNAlater made of?
Actually, RNALater is just a near-saturated Ammonium Sulfate solution buffered with Sodium Citrate, and with some EDTA for metal chelation.
Does RNAlater preserve DNA?
Thus, RNAlater successfully preserved both RNA and DNA for up to 1 month at room temperature, and up to 4 months at 5°C, providing an alternative to the deep freezing. This method will enable a greater integration of molecular methods in ecological studies.
How long does RNAlater last?
Once in RNAlater, samples can be stored for up to 1 day at 37°C, 1 week at 25°C, 1 month or more at 4°C, and long-term at -20°C or -80°.
How long can you store RNAlater?
Most samples can be stored in RNAlater® Solution at 4°C for up to 1 month without significant RNA degradation.
Does RNAlater preserve protein?
RNAlater is a valuable fixative used for preservation of biological samples. The solution denatures proteases and RNases to preserve RNA and protein integrity for downstream analysis.
How do I store my RNAlater?
Do not freeze samples in RNAlater® Solution immediately; store at 4°C overnight (to allow the solution to thoroughly penetrate the tissue), remove supernatant, then move to –20°C or –80°C for long-term storage. Note: We offer RNAlater®-ICE (P/N AM7030) to recover tissues that have already been frozen.
How do you store TRIzol?
Resuspend the pellet in 1 mL of 75% ethanol per 1 mL of TRIzol™ Reagent used for lysis. Note: The RNA can be stored in 75% ethanol for at least 1 year at –20°C, or at least 1 week at 4°C.
How do you preserve tissue for RNA extraction?
The RNA is considered fragile, and the recommendation is to flash-freeze the samples in liquid nitrogen, to preserve them at − 70 °C or lower, and to avoid freeze-thaw cycles both of the tissue sample and of the extracted RNA (Sambrook and Russell 2001).
Can you freeze RNAlater?
Do not freeze samples in RNAlater® Solution immediately; store at 4°C overnight (to allow the solution to thoroughly penetrate the tissue), remove supernatant, then move to –20°C or –80°C for long-term storage.
Is RNAlater toxic?
According to the manufacturer the lack of RNAlater toxicity makes the product user-friendly for sample transportation, but biological safety may still be a concern with some RNAlater-treated specimens. Picornavirus, rhabdovirus, and HIV in cell culture supernatants retain infectivity after RNAlater stabilization(5).
Does the use of RNAlater RNA stabilization solution affect the outcome?
In-house research, as well recently published independent research, indicates that the use of RNAlater RNA Stabilization Solution for tissue storage does not affect the outcome of subsequent RNA expression analysis experiments compared to other processing methods. For Research Use Only. Not for use in diagnostic procedures.
Which MagMax kits are compatible with RNAlater reagent?
Only MagMax96 for Microarrays Total RNA Isolation Kit (Cat. No. AM1839) is compatible, since it uses Trizol reagent and most of the salt carry-over from RNAlater reagent is eliminated during the lysis steps and partitioned from the aqueous RNA layer on top. For other MagMax kits, optimization is needed.
Are RNAlater solution–preserved samples suitable for histology?
RNAlater solution–preserved samples are suitable for histology and provide excellent morphological detail. Sections made from RNAlater-preserved material were indistinguishable from slides made from untreated samples when examined for standard histological criteria. (A).
Can I sort cells by fluorescence-activated cell sorting after using RNAlater stabilization solution?
Yes, you should be able to sort cells by fluorescence-activated cell sorting (FACS) after treatment with RNAlater Stabilization Solution. If you run into problems due to the viscosity of RNAlater Stabilization Solution, you may need to dilute it with cold nuclease-free water.