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What is NcoI restriction enzyme?

What is NcoI restriction enzyme?

NcoI (MB066) Description: NcoI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the NcoI gene from Nocardia corallina. NcoI activity is not affected by dam methylation, dcm methylation or CpG methylation. NZYTech’s NcoI is an enzyme that acts at 37 °C.

What type of enzyme is NcoI?

DNA Restriction Enzymes
DNA Restriction Enzymes from Takara such as NcoI are high-quality: perform restriction enzyme digestion with reliable restriction endonucleases.

What is star activity restriction enzymes?

Star activity is the relaxation or alteration of the specificity of restriction enzyme mediated cleavage of DNA that can occur under reaction conditions that differ significantly from those optimal for the enzyme.

What is double digestion with restriction enzymes?

A double digest is one where two restriction enzymes are used to digest DNA in a single reaction. In this case you will be using EcoR I and BamH I. There is only one site in the plasmid vector for each of these enzymes and they are located on either side of your insert DNA.

What does NCOI mean?

Definition. NCOI. National Committee on Inquiry (National Association of Social Workers)

Why is it called star activity?

It has been demonstrated that under extreme non-standard conditions, restriction endonucleases are capable of cleaving sequences which are similar but not identical to their defined recognition sequence. This altered or relaxed specificity has been termed “”star”” activity.

What is the difference between single and double digest?

Single-digested plasmid refers to a plasmid digested by a single restriction enzyme while double-digested plasmid refers to a plasmid digested by two different restriction enzymes.

What are neoschizomers & isoschizomers and Isocaudomers?

Isoschizomers are the restriction enzymes which recognize and cleave at the same recognition site. For example, SphI (CGTAC/G) and BbuI (CGTAC/G) are isoschizomers of each other. Neoschizomers are the restriction enzymes which recognize the same site and have a different cleavage pattern.

How do neoschizomers differ from isoschizomers?

The key difference between isoschizomers and neoschizomers is that isoschizomers are restriction enzymes that have the same recognition sequence and cleave the DNA at the same positions, while neoschizomers are restriction enzymes that have the same recognition sequence but cleave DNA at different positions.

How can we reduce star activity?

To prevent star activity, we recommend the following guidelines: Use as few units of restriction enzyme as possible for a complete digestion, which avoids overdigestion of the DNA and reduces the final glycerol concentration in the reaction.

What is Invitrogen TRIzol reagent?

Product information. Invitrogen ™ TRIzol Reagent is a ready-to-use reagent, designed to isolate high quality total RNA (as well as DNA and proteins) from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, within one hour.

What are the advantages of TRIzol reagent over other methods?

The simplicity of the TRIzol Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in 1 hour. Total RNA isolated by TRIzol Reagent is free of protein and DNA contamination.

How is total RNA isolated by TRIzol reagent?

Total RNA isolated by TRIzol® Reagent is free of protein and DNA contamination. Formulated for isolation of multiple molecular targets. TRIzol® Reagent allows you to perform sequential precipitation of RNA, DNA, and proteins from a single sample. After homogenizing the sample with TRIzol® Reagent, chloroform is added,

What is the maximum sample volume for TRIzol lysis?

Note: The sample volume should not exceed 10% of the volumeof TRIzol™ Reagent used for lysis. STOPPING POINTSamples can be stored at 4°C overnight or at –20°C for up to a year. (Optional) If samples have a high fat content, centrifuge the lysate for 5 minutes at 12,000 × supernatant to a new tube.