What is gel filtration chromatography using a column?
Gel Filtration Chromatography Mechanism In a gel filtration chromatography column, the stationary phase is composed of a porous matrix, and the mobile phase is the buffer that flows in between the matrix beads. The beads have a defined pore size range, known as the fractionation range.
What are the principles and applications of gel filtration?
Gel filtration chromatography (sometimes referred to as molecular sieve chromatogra- phy) is a method that separates molecules according to their size and shape. The sepa- ration of the components in the sample mixture, with some exceptions, correlates with their molecular weights.
What happens in a gel filtration column?
A gel filtration column separates molecules and complexes by size. The separation is not strictly by molecular mass, since the shape of the molecule or complex can affect migration through the column.
What is the action principle of gel filtration chromatography?
Gel-Filtration Chromatography Gel filtration is also known as size-exclusion chromatography or molecular-sieve chromatography. In this process, separation is based on the differing ability (due to differing molecular size) of molecules in the sample to enter the pores of the gel-filtration medium.
What are the advantages of gel-filtration?
One of the principal advantages of gel-filtration chromatography is that separation can be performed under conditions specifically designed to maintain the stability and activity of the molecule of interest without compromising resolution.
What are the advantages of gel filtration?
What is Rf value in chromatography?
What is RF Value? The Rf (retardation factor) value is the ratio of the solute’s distance travelled to the solvent’s distance travelled. The word comes from chromatography, when it was discovered that a given component will always travel the same distance in a given solvent under the same conditions.
What is the HPLC principle?
Principle of HPLC The specific intermolecular interactions between the molecules of a sample and the packing material define their time “on-column”. Hence, different constituents of a sample are eluted at different times. Thereby, the separation of the sample ingredients is achieved.
What are the detectors used in HPLC?
Lesson 6: Detectors for HPLC
- UV, VIS, and PDA Detectors. Refractive-Index Detector.
- UV, VIS, and PDA Detectors.
- Refractive-Index Detector.
- Evaporative Light Scattering Detector.
- Multi-Angle Light Scattering Detector.
- Mass Spectrometer.
- Conductivity Detector.
- Fluorescence Detector.