What is anion exchange resin column?
Anion Exchange Column Design Ion exchange chromatography is used to separate charged molecules. In an anion exchange column, the packing is positively charged and therefore retains negatively charged molecules by coulombic interaction. The bound molecules are eluted with an anion gradient.
What is anion exchanger?
Definition of anion exchanger : an anion-exchange agent that can exchange its anion with the anion or anions of a solution passed through it or that can adsorb anions in the form of acids especially : an insoluble basic synthetic organic resin usually containing amine groups or quaternary ammonium groups.
What binds to a cation exchange resin?
Generally speaking, a protein will bind to a cation exchange resin if the buffer pH is lower than the isoelectric point (pI) of the protein, and will bind to an anion exchange resin if the pH is higher than the pI.
What is used as ion exchange resin in ion exchange chromatography?
Anion resins and cation resins are the two most common resins used in the ion-exchange process. While anion resins attract negatively charged ions, cation resins attract positively charged ions.
How does anion exchange resin work?
Put simply, ion exchange is a reversible interchange of charged particles—or ions—with those of like charge. This occurs when ions present on an insoluble IX resin matrix effectively swap places with ions of a similar charge that are present in a surrounding solution.
What is the principle of anion exchange chromatography?
Anion-exchange chromatography is a process that separates substances based on their charges using an ion-exchange resin containing positively charged groups, such as diethyl-aminoethyl groups (DEAE). In solution, the resin is coated with positively charged counter-ions (cations).
Which functional group is present in anion exchange resin?
The functional groups are sulfonic (–SO3H), carboxylic (–COOH), or phosphonic (–PO32 −) acids for resins capable of exchanging cations, whereas anion exchange resins possess basic functional groups, such as quaternary ammonium or ammine groups (Korkisch, 1989).
What are chromatography resins made of?
Resins are usually made from the polystyrene polymer backbone and differ only by their specific functional groups. The ion exchange systems are usually regenerated with sodium chloride. The strength of the solution depends on the strength of the adsorption bond.
What material of resin is required for ion exchange chromatography?
Resin Selection in Ion Exchange Chromatography Matrices are usually made of cellulose, polystyrene, agarose, and polyacrylamide. Some of the factors affecting resin choice are anion or cation exchanger, flow rate, weak or strong ion exchanger, particle size of the resin, and binding capacity.
Which polymer is used for preparation of ion exchange resin?
Amination of vinylbenzyl chloride-divinylbenzene (VBC-DVB) copolymers is an effective method for preparation of ion-exchange resins. Conventionally, the starting polymer is produced by chloromethylation of a styrene-divinylbenzene copolymer that utilizes chloromethyl methyl ether, a known carcinogen.
Which buffer is used for anion exchange chromatography?
Buffer preparation Buffer counterions should have the same charge as the resin; for positively charged anion exchange resins, Tris buffers are an excellent choice.
What binds to an anion exchange column?
Anion exchange resins will bind to negatively charged molecules, displacing the counter-ion. Anion exchange chromatography is commonly used to purify proteins, amino acids, sugars/carbohydrates and other acidic substances with a negative charge at higher pH levels.