What are the target genes of beta-catenin?
β-catenin translocates to the nucleus and interferes with the TCF- LEF co-transcription factors. This leads to the stimulation of several β-catenin target genes (CYCLIN D1, c-MYC, PDK, MTC-1, MMP7, fibronectin, COX-2, AXIN-2) (7, 8, 21, 22) (Figure 2).
What genes does B catenin activate?
In the nucleus β-catenin activates transcription of Wnt/β-catenin-target genes such as c-myc and cyclinD1 (Maretzky et al, 2005; Reiss et al, 2005).
What is the role of B catenin?
Beta-catenin (β-catenin) is a multifunctional, 90 kD protein that contributes to cell development under normal physiological conditions [55]. β-Catenin is a crucial transcriptional factor in Wingless–Int (Wnt) signaling, and plays important role in stem cell renewal and organ regeneration [56].
What does beta-catenin bind to?
Beta-catenin is a dual function protein, involved in regulation and coordination of cell–cell adhesion and gene transcription. In humans, the CTNNB1 protein is encoded by the CTNNB1 gene. In Drosophila, the homologous protein is called armadillo….Catenin beta-1.
| RNA expression pattern | |
|---|---|
| BioGPS | More reference expression data |
What is a destruction complex?
The destruction complex is likely a dynamic multiprotein assembly, but its core components include, in addition to β-catenin itself, the Ser/Thr kinases glycogen synthase kinase 3 (GSK-3) and casein kinase 1 (CK1), the scaffolding protein Axin, the adenomatous polyposis coli (APC) protein, and the E3-ubiquitin ligase β …
Does beta catenin bind DNA directly?
TCF/LEF factors directly bind to DNA and mediate the transcriptional response elicited by Wnt/β-catenin. Studies in other developmental systems have generally documented repressive roles for Tcf7l1 and Tcf7l2, and activating roles for Tcf7 and Lef1 (Lien and Fuchs, 2014).
How is beta catenin regulated?
β-Catenin is a pivotal component of the Wnt signaling pathway and it is tightly regulated at three hierarchical levels: protein stability, subcellular localization and transcriptional activity.
How does beta-catenin affect Wnt signaling?
In turn, Wnt/β-catenin signaling induces the expression and activity of β-TrCP ubiquitin ligase receptor and this negative feedback loop controls the β-catenin/TCF4 pathway [39]. Moreover, induction of the β-TrCP–GSK3β interaction is required for late-phase stabilization of β-catenin in canonical Wnt signaling.
How does the Wnt B catenin pathway work?
The Wnt/β-catenin pathway is activated when a Wnt ligand binds to a seven-pass transmembrane Frizzled (Fz) receptor and its co-receptor, low-density lipoprotein receptor related protein 6 (LRP6) or its close relative LRP5.
Does beta-catenin bind DNA directly?
What is beta catenin destruction complex?
The beta-catenin destruction complex plays a key role in the canonical Wnt signaling pathway. In the absence of Wnt signaling, this complex controls the levels of cytoplamic beta-catenin. Beta-catenin associates with and is phosphorylated by the destruction complex.
How is beta catenin degraded?
Ubiquitinated β-catenin is degraded by the proteasome. The molecular mechanisms that underlie several aspects of destruction complex function are poorly understood, particularly the role of APC.