Discover the world with our lifehacks

How does TOF mass spectrometry work?

How does TOF mass spectrometry work?

A common form of mass spectrometry is time of flight (ToF) mass spectrometry. In this technique, particles of the substance are ionised to form 1+ ions which are accelerated so that they all have the same kinetic energy. The time taken to travel a fixed distance is then used to find the mass of each ion in the sample.

What is MALDI-TOF mass spectrometry used for?

Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has become a widely used technique for the rapid and accurate identification of bacteria, mycobacteria and certain fungal pathogens in the clinical microbiology laboratory.

What is MALDI in mass spectrometry?

In mass spectrometry, matrix-assisted laser desorption/ionization (MALDI) is an ionization technique that uses a laser energy absorbing matrix to create ions from large molecules with minimal fragmentation.

What molecule does MALDI-TOF identify?

After the matrix solution air-dried, the spotted samples were analyzed by MS (Jeong et al., 2013, 2014). MALDI-TOF MS has also been shown to be useful for detection of protein toxins, such as staphylococcal enterotoxin B, botulinum neurotoxins, Clostridium perfringens epsilon toxin, shiga toxin etc.

How are ions formed in a TOF mass spectrometer?

Time of Flight (TOF) Mass Spectrometer Electrospray Ionisation: The sample is dissolved in a volatile solvent and injected through a fine hollow needle at high pressure. As the solvent evaporates it is subjected to a high voltage which forms positive ions (because it loses electrons).

What is being detected in mass spectrometry?

Typically, mass spectrometers can be used to identify unknown compounds via molecular weight determination, to quantify known compounds, and to determine structure and chemical properties of molecules.

What is MALDI-TOF and how does it work?

During MALDI-TOF analysis, the m/z ratio of an ion is measured by determining the time required for it to travel the length of the flight tube. A few TOF analyzers incorporate an ion mirror at the rear end of the flight tube, which serves to reflect back ions through the flight tube to a detector.

How is MALDI-TOF performed?

MALDI-TOF measures the mass of molecules from a sample that has been embedded in a matrix by using a laser to ablate and desorb the molecules with minimal fragmentation.

How many organisms can MALDI-TOF identify?

For Gram-negative bacteria evaluation, there are 2263 clinical significant species can be analyzed by MALDI-TOF MS identification.

What type of test is MALDI-TOF?

Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) is a powerful analytical mass spectrometry technique that has generated numerous diagnostic and clinical applications, especially for the identification of microorganisms for medical diagnosis.

How is relative abundance measured in a TOF mass spectrometer?

The relative abundance for a specific ion in the sample can be calculated by dividing by the number of ions with a particular m / z m/z m/z ratio by the total number of ions detected. At the end of the experiment, the instrument generates a mass spectrum for the sample, which plots relative abundance vs. m/z .

Why are particles ionised in mass spectrometry?

Ionization Because mass spectrometry measures the mass of charged particles, only ions will be detected, and neutral molecules will not be seen. Ions are created by giving electrons to a molecule (producing a negatively charged ion) or taking electrons away from a molecule (producing a positively charged ion).