How does plaque size relate to properties of your phage?
Usually phage infection is studied in a layer of soft agar (or “top agar”) which allows the phage to diffuse rapidly. The size of the plaque is proportional to the efficiency of adsorption, the length of the latent period, and the burst size of the phage.
Why do phage plaques not continue to grow and expand in size?
Because phage can only reproduce in actively growing cells, the size of the plaques produced will depend on how soon the bacteria in the agar reach stationary phase and stop reproducing. Plaques will stop spreading at this point.
What are phage plaques?
A phage plaque is a clearing in a bacterial lawn. Plaques form via an outward diffusion of phage virions that is fed by bacterial infection. Anything that slows phage diffusion can impede plaque development and thereby plaque size.
How are bacterial colonies and phage plaques different quizlet?
How are bacterial colonies and phage plaques different? Bacterial colonies are observable growth whereas phage plaques are areas without growth. When calculating the number of phage per milliliter in the original phage solution, the areas of bacterial destruction, or plaques, on the plates have to be counted.
How are bacterial colonies and phage plaques different?
Simple Difference: Colony refers to a cluster of particular bacteria developed in a medium. Plaque refers to a clear zone, produced by a Phage which is formed by lysis of the bacterial cells in the medium.
What factors influence plaque development?
Factors influencing the size of plaque were as follows; pH of medium, agar concentration and amount of overlayer, number of indicator cell, incubation temperature of plate, and addition of some reducing compounds (ascorbate, cysteine, formate, sulfide and thioglycollate) to medium.
Will the plaques get bigger in size if we incubate the plates for longer time?
Plague size depends on the phage more than the incubation period. Usually, you can achieve the best plague size in 24-48 hours of incubation until your phage has a long latent period. From my experience, a Longer period of incubation will diminish your plague.
What is a colony in bacteria?
A bacterial colony is what you call a group of bacteria derived from the same mother cell. This means that a single mother cell reproduces to make a group of genetically identical cells, and this group of cells form a mass, which is known as a bacterial colony.
What are the 5 steps in the lytic cycle of virus reproduction?
Following are the steps of lytic cycle..
- Attachment: In this step, the bacteriophage, attaches itself by it’s tail to the.
- Digestion: In this step, the bacteriophage contains an enzyme called.
- Injection:
- Taking Control:
- Multiplication:
- Rupturing:
What is the difference between a plaque and a colony?
What causes the formation of plaques in a bacteriophage assay?
The spread of infectious phage from the initially infected bacterial cell to the surrounding cells results in the lysis of the bacteria in the vicinity, eventually forming the plaque that is large enough to be visible to the naked eye. Plaques do not continue to spread indefinitely.
How do bacteria colonies grow?
As the bacteria consume the nutrients, they begin to grow and multiply. This generates thousands to millions to billions of cells that begin to pile up, becoming visible to the naked eye. This pile of cells originates from one cell and is called a bacterial colony.
Is phage growth as plaque a useful technique for phage studies?
Second, phage growth as plaques is a central technique to phage studies, yet appears to be under appreciated by phage workers in terms of its complexity. Third, selective pressures acting on phage during plaque growth differ from those seen during broth growth.
How to characterize bacteriophage plaques?
Bacteriophage plaques: theory and analysis Laboratory characterization of bacteriophage growth traditionally is done either in broth cultures or in semisolid agar media. These two environments may be distinguished in terms of their spatial structure, i.e., the degree to which they limit diffusion, motility, and environmental mixing. Well-mix …
Does bacterial growth rate affect phage growth rate?
The results clearly showed that bacterial growth rate has an important influence on all three phage growth parameters, namely adsorption constant, latent period and burst size, which together determine the bacteriophage population growth rate.
When were phages first used to treat bacterial infections?
Using phages to treat bacterial infections was developedback in the 1920s and 1930s in Eastern Europe and the Soviet Union. Plaque assay is one of the widely used approaches for determining the quantity of infectious virus in a sample.