How do rats induce streptozotocin in diabetes?
For induction of experimental diabetes in male adult rats weighted 250-300 grams (75-90 days), 60mg/kg of Streptozotocin was injected intravenously. Three days after degeneration of beta cells, diabetes was induced in all animals.
How do you make a streptozotocin solution?
Streptozotocin Injections For 100 ml, combine 1.05 g citric acid and 1.48 g sodium citrate in dH20. Ensure pH is 4.5, then bring up to 100 mL with water.
What type of diabetes does streptozotocin induce?
Abstract. Streptozotocin (STZ) is an antibiotic that produces pancreatic islet β-cell destruction and is widely used experimentally to produce a model of type 1 diabetes mellitus (T1DM).
Why streptozotocin is used?
Streptozotocin, produced by Streptomyces achromogenes, is an antineoplastic agent approved by US FDA in 1982 for the treatment of metastatic cancers of pancreatic islet of langerhans. It is an alkylating agent and has been reported to have antibacterial activities [117].
How do you inject streptozotocin in rats?
Inject an equal volume of citrate buffer (pH 4.5) intraperitoneally into the control animals. This dose of STZ should produce a stable hyperglycemia in the high-fat diet rat for at least 130 days. The dose of STZ is critical.
What is the difference between alloxan and streptozotocin?
strptozotocin has high inductive capacity than alloxan and its less toxic to the pancreas compared to alloxan . It is also more specific to the pancreas while alloxan in addition to being specific to the pancreas also appears toxic to some other organs.
How will you prepare 0.1 M citrate buffer ph4 5?
- 0.1 M Citrate Buffer Preparation: Mix 0.1 M Sodium Citrate and 0.1 M Citric acid. Adjust pH to 4.5 with 0.1 M Citric acid. Dissolve streptozotocin in Sodium Citrate Buffer. Cite. 1 Recommendation.
- 0.1 M citric acid. Dissolve 1.05 g of citric acid monohydrate (Sigma C1909, MW 210.14) in 50 ml ddH2O and store at.
How do rats induce type 2 diabetes?
Methods: Wistar rats were fed with a high-fat diet for 8 weeks followed by a one-time injection of 25 or 35 mg/kg streptozotocin to induce type 2 diabetes. Then the diabetic rats were fed with regular diet/high-fat diet for 4 weeks. Changes in biochemical parameters were monitored during the 4 weeks.
How does alloxan induce diabetes in rats?
Anti-diabetic Activity in Alloxan Induced Diabetes Model Diabetes was induced in 80 male albino rats by injecting 120 mg/kg of Alloxan monohydrate intra-peritoneally dissolved in 0.9% w/v cold normal saline to overnight-fasted rats (12 h).
Why is streptozotocin better than alloxan?
Streptozotocin caused an increase in mechanical sensitivity in both hyperglycemic and normoglycemic rats, while alloxan induced mechanical sensitization only in hyperglycemic animals.
How do you calculate citrate buffer?
- Prepare 800 mL of distilled water in a suitable container.
- Add 25.703 g of Sodium Citrate dihydrate to the solution.
- Add 2.421 g of Citric Acid to the solution.
- Adjust solution to final desired pH using HCl or NaOH.
- Add distilled water until the volume is 1 L.
How do you make a 0.2 M citrate buffer?
(vii) 0.2M Citrate buffer (pH 5.0): Add 20.5 ml of 0.1M citric acid to 29.5 ml of 0.1M sodium citrate (C6H5O7Na3. 2H2O) and dilute to 100 ml with distilled water.