What is the function of cauliflower mosaic virus CaMV in the production of recombinant proteins?
Cauliflower mosaic virus (CaMV) upregulates translation reinitiation of its pregenomic polycistronic 35S RNA via interaction with the cell’s translation machinery.
How to control Cauliflower mosaic virus?
There is no cure for cauliflower mosaic virus. Once a plant is infected, then it is too late. Prevention is the only way to prevent agricultural or gardening losses. Since cauliflower mosaic virus is usually spread by aphids, so any sort of pest control can be beneficial in reducing infection.
What is the genome of Cauliflower mosaic virus?
The CaMV genome consists of approximately 8,000 base-pairs of circular, double-stranded DNA. The genome encodes seven genes (gene I to gene VII), also called P1 to P7 for encoded proteins 1-7.
What is CaMV 35S?
The cauliflower mosaic virus promoter (CaMV 35S) is used in most transgenic crops to activate foreign genes which have been artificially inserted into the host plant. It is inserted into transgenic plants in a form which is different from that found when it is present in its natural Brassica plant hosts.
Why is CaMV a good promoter?
The 35S CaMV promoter is generally considered to be a strong constitutive promoter1 and it facilitates high level of RNA transcription in a wide variety of plants, including plants well outside the host range of the virus2.
What is 35S promoter and NOS terminator?
The 35S promoter (p35S) and NOS terminator (tNOS) are both transcription-regulating sequences [9, 10]. To date, many EU-authorized GMOs (17/24) contain either the “p35S” (15/24) or the “tNOS” (15/24) or both (9/24) [8, 11, 12] (for more details see Table 1).
How can we prevent mosaic virus?
How to Prevent Mosaic Viruses
- Plant virus-resistant varieties in your garden.
- Mosaic viruses are mostly spread by insects, especially aphids and leafhoppers.
- Control your weeds.
- To avoid seed-borne mosaic viruses, soak seeds of susceptible plants in a 10% bleach solution before planting.
What does the 35S promoter gene do?
What is the role of 35S promoter?
Why is the CaMV 35S promoter used in making GMO plants instead of the native promoter of the gene of interest?
The most common promoter used in GM crops is the 35S promoter from the cauliflower mosaic virus (CaMV 35S). This promoter is chosen because it is already designed by nature to activate transcription in all plant cell types.
What is the purpose of 35S?
What does nos terminator do?
tumefaciens) NOS terminator. The control test detects a region of the A. tumefaciens genome that is not used in the genetic modification of plants. This kit is recommended for use with genesig Easy DNA/RNA extraction kit.