What is TaqMan array?
TaqMan Array Cards are high-throughput, accurate, sensitive, and simple-to-use tools for quantitative analysis of mRNA or miRNA transcripts using a real-time PCR protocol. They utilize a microfluidic card with 384 reaction chambers and eight sample loading ports.
What is FAM TaqMan?
Recommendations. Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5′ end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3′ end.
Do TaqMan probes expire?
Each gene-specific assay consists of a singe tube containing a fluorescently labeled TaqMan® MGB probe, a forward primer, and a reverse primer. The shelf life of these assays has recently been extended to five years—three years beyond the original control date.
Can we use TaqMan for duplex PCR?
Summary Applied Biosystems™ TaqMan® Gene Expression Assays provide excellent duplex qPCR performance, with results that are highly correlated with those obtained from the same assays run in singleplex.
What is TaqMan Low Density Array?
TaqMan low-density array (TLDA) cards (Applied Biosystems, Foster City, CA) are 384-well microfluidic cards with eight ports, each containing 48 connected wells (Fig. 1). The primers and probe for each assay are preloaded and dried onto the designated duplicate wells.
What is a quencher in PCR?
The quencher molecule quenches the fluorescence emitted by the fluorophore when excited by the cycler’s light source via Förster resonance energy transfer (FRET). As long as the fluorophore and the quencher are in proximity, quenching inhibits any fluorescence signals.
What is the difference between SYBR Green and TaqMan?
The main difference between STBR Green and Taqman is that SYBR green is a dsDNA binding dye used to detect PCR products accumulated during the PCR reaction whereas Taqman is a fluorogenic probe specific to a target gene, which accumulates during PCR.
What is the quencher in TaqMan?
Is TaqMan a trademark?
TaqMan is a registered trademark of Roche Molecular Systems, Inc. Roche sold the license to Applied Biosystems to be used in RESEARCH APPLICATIONS. Only Roche owns and use the license in Diagnostics.
What is duplex RT PCR?
A duplex qPCR method allows the simultaneous detection of two viruses in the same sample, which is more cost-effective than assaying for each virus separately.
What is MGB probe?
Applied Biosystems TaqMan MGB (minor groove binder) probes are dual-labeled probes used for real-time PCR applications using TaqMan chemistry. TaqMan MGB Probes incorporate a 5′ fluorescent reporter dye and a 3′ nonfluorescent quencher (NFQ).
What is low density array?
LDA technology allows the simultaneous measurement of expression of up to 384 genes in a single sample. Each array has eight channels, allowing between one to eight cDNA samples to be loaded. Each channel services 48 wells, with the possibility of having all 48 wells containing different primer probe sets.