How do you select a column in gas chromatography?
The selection of the proper capillary column for any application should be based on four significant factors: stationary phase, column I.D., film thickness, and column length. The practical effects of these factors on the performance of the column are discussed briefly in this section, in order of importance.
What are typical dimensions for a capillary GC column?
As there was no standardization for some time, capillary columns with different IDs showed up. The ones that are mostly used are: 0.22 mm, 0.20 mm, 0.18 mm, 0.15 mm and 0.10 mm. If you look at the supplier of fused silica tubing, there are even more diameters available [1].
How long is a GC column?
15 m to about 60 m.
Normally capillary GC columns range in length from 15 m to about 60 m. Such column lengths are generally sufficient to resolve complex compounds having closely spaced or even overlapping peaks.
What is film thickness in GC column?
columns have a 0.25 or 0.50 µM film thickness.
Why GC columns are longer than HPLC?
A gas encounters less resistance in the GC column which permits use of longer column lengths. Sample stability – samples analysed by HPLC are generally thermally labile so they are in liquid phase in the HPLC column at room temperatures.
What are C18 columns?
C18 columns are HPLC (high performance liquid chromatography) columns that use a C18 substance as the stationary phase. C18 HPLC columns are used in environmental sciences and chemical analysis, as well as industries such as pharmaceutical and environmental sciences, to analyze individual parts of chemical mixtures.
What is DB in GC column?
DB-1 Columns Agilent J&W DB-1 is nonpolar and low-bleed, and operates under high temperature limits. It is an excellent choice for general purpose use in a wide variety of applications. Precision-engineered DB-1 is bonded, crosslinked and solvent rinsable, and is equivalent to USP phase G2.
Do GC columns expire?
A related question that we hear quite often is “what is the shelf life of a capillary GC column.” Once again, there are no right or wrong answers and GC columns usually don’t ship with an expiration date on them – but in general, a pretty good guideline guide line is to use the column within about two years to ensure …
What is SPB®-1 capillary GC column?
Limits: SPB®-1 Capillary GC Column was used for speciation of the nitrogen compounds (quantitatively), which are polar and somewhat basic in nature, with chemiluminescence detection.
What kind of column is used for speciation?
Temp. Limits: SPB®-1 Capillary GC Column was used for speciation of the nitrogen compounds (quantitatively), which are polar and somewhat basic in nature, with chemiluminescence detection.
What is the USP code for analytic column?
Analytes will be separated primarily according to boiling point. USP Code: This column meets USP G1, G2, and G9 requirements. Temp. Limits: SPB®-1 Capillary GC Column was used for speciation of the nitrogen compounds (quantitatively), which are polar and somewhat basic in nature, with chemiluminescence detection.
What is the USP code for a non polar column?
Application: This column is often used for traditional general purpose applications, where a non-polar column is required. Analytes will be separated primarily according to boiling point. USP Code: This column meets USP G1, G2, and G9 requirements. Temp.