How much does aptamer cost?
For example, if you have 16 targets, your price can be as little as $5000 per custom aptamer. All prices include quantitative binding validation (Kd determination) and access to monoclonal aptamer material to validate in your assay. Additional aptamer material can be purchased for approximately $200-500/100μg.
How do I buy an aptamer?
How to order aptamers. To order aptamers online, visit the Custom DNA Oligos or Custom RNA Oligos ordering pages. Enter your desired scale, purification, and the sequence(s) with random bases or modifications to suit your needs.
How does an aptamer work?
Aptamers are single-stranded oligonucleotides that fold into defined architectures and bind to targets such as proteins. In binding proteins they often inhibit protein–protein interactions and thereby may elicit therapeutic effects such as antagonism.
How do I make an aptamer?
Two major steps exist in aptamer design selection and optimization. In the first step, several polynucleotides with probable binding affinity toward a target are screened by using the SELEX method and then selected. In the second step, aptamers with detected high affinity are shortened, modified, and stabilized.
What is DNA aptamer?
Aptamers are a special class of nucleic acid molecules that are beginning to be investigated for clinical use. These small RNA/DNA molecules can form secondary and tertiary structures capable of specifically binding proteins or other cellular targets; they are essentially a chemical equivalent of antibodies.
What is the size of aptamer?
Aptamers are small (usually from 20 to 60 nucleotides) single-stranded RN A or DNA oligonucleotides able to bind target molecules with high affinity and specificity.
What are aptamers used for?
Like monoclonal antibodies, aptamers can be used for the molecular recognition of their respective targets. Aptamers have been successfully used for pathogen recognition, cancer recognition, monitoring environmental contamination, and as stem cell markers.
What is the purpose of SELEX?
SELEX is a powerful method to isolate RNA sequences that bind to particular substrates without necessarily reacting with them. Random pools of RNA, typically 40–100 nucleotides in length, are created such that billions of possible sequences are represented.
What is an aptamer how is it selected and how is it used as a therapeutic agent?
Aptamers are short oligonucleotides of DNA or RNA selected in vitro to bind a specific target with high specificity and high affinity which require simple synthesis protocols compared to the in vivo development of antibodies.
What is a peptide aptamer?
Peptide aptamers are small combinatorial proteins that are selected to bind to specific sites on their target molecules. Peptide aptamers consist of short, 5-20 amino acid residues long sequences, typically embedded as a loop within a stable protein scaffold.
What is the difference between aptamer and antibody?
Aptamers are produced through a simple and inexpensive process and the time required to generate aptamers is comparatively short. Unlike antibodies, aptamers do not need animals or an immune response for their production.
What is aptamer screening?
Aptamers are a class of single oligonucleotide molecules (DNA or RNA) that are screened from random DNA or RNA oligonucleotide chain libraries by the systemic evolution of ligands by exponential enrichment technology.
Is there a database for aptamers?
We offer the Apta-index, the most advanced user-friendly database on aptamers. Aptagen does not list this information contained herein as products but as a database of information obtained from the published literature. If your lab has developed aptamer (s), we would like to hear from you.
How do you identify aptamers for nucleic acids?
Nucleic acid aptamers are often identified using an iterative enrichment technique, such as SELEX (Figure 2), where oligos or proteins with increased affinity and specificity to a target molecule are isolated from a sequence pool after several rounds of selection. Figure 1. Engineering aptamers to bind specific targets.
What is the KDR range for DNA aptamers?
This technique has produced DNA aptamers for several small-molecule targets, with KDranging from 1–2,000 nM after 5–10 rounds. [98–101]Another homogenous technique, sol-gel SELEX, utilizes a microfluidic device that incorporates a silicon chip spotted with droplets of sol-gels containing the target.
How are aptamers made and tested?
As an easy way to generate aptamer designs in the laboratory for initial testing, scientists will sometimes in vitro transcribe RNA aptamers. When doing this, they often include 2′-Fluoro pyrimidine base modifications to improve aptamer stability.