What is RI detector in HPLC?
HPLC Refractive Index Detectors (HPLC RI Detectors) are used with high-pressure liquid chromatographs when detecting substances with limited or no UV absorption. These chemical components included alcohols, sugars, fatty acids, polymers and carbohydrates.
Which type of detector are used in HPLC?
UV, VIS, and PDA Detectors UV detector is a very commonly used detector for HPLC analysis. During the analysis, sample goes through a clear color-less glass cell, called flow cell.
What is sensitivity in RI detector?
A refractive index detector is a universal detector since it monitors a bulk property of the solution. Unfortunately, RI detectors have a very low sensitivity (mg/L range) and are subject to several caveats: RI detectors are very sensitive to changes in temperature. Both the detector and column must be thermostatted.
What is a refractive index detector?
A refractive index (RI) detector, also called a differential refractometer, measures refractive index when light is passed through an analyte-containing cell as compared to a reference cell containing just solvent.
What is USP tailing factor?
The Tailing Factor is defined by the USP as the distance from the front edge of the peak to the back edge, divided by the distance from the front edge to the centerline, with all distances measured at 5% of the maximum peak height.
What is the range of UV detector?
A UV detector employs a deuterium discharge lamp (D2 lamp) as a light source, with the wavelength of its light ranging from 190 to 380 nm. If components are to be detected at wavelength longer than this, a UV-VIS detector is used, which employs an additional tungsten lamp (W lamp). Figure 1 shows the optical system.
Why RI detector is called universal detector?
They are considered to be universal detectors because they can detect anything with a refractive index different from the solvent, but they have low sensitivity.
How do you stabilize a RI detector?
Follow these thirteen points to optimize the performance of your refractive index detector.
- Position the solvent and waste reservoirs correctly.
- Do not over pressurize the flow cell.
- Use the correct solvents.
- Check for leaks.
- Verify frit, filter and fitting quality.
- Control the optical unit temperature.