What is multiplex PCR used for?
Multiplex PCR is used in life science research, clinical diagnostics, and forensic laboratories. The development of PCR detection systems with simultaneous multi-target detection and advances in probe chemistries have made comparative analyses standard in many areas of research and testing.
What is multiplex tandem PCR?
Multiplex Tandem PCR (MT-PCR) is a unique patented technology that allows the detection of multiple targets in one sample without compromising analytical sensitivity and specificity.
How is PCR different from multiplex PCR?
In conventional singleplex PCR, a single target is amplified in a single reaction tube. In contrast, multiplex PCR allows for simultaneous amplification of multiple target sequences in a single tube using specific primer sets in combination with probes labeled with spectrally distinct fluorophores.
What is multiplex PCR used for in forensics?
Multiplex polymerase chain reaction is a technique by which different fragments of DNA are amplified and used for detection of large mutations depending on their three-dimensional shape. DNA sequencing (Fig. 2-84) is used to determine the sequence of nucleotides and to detect mutations and their type.
Is multiplex PCR expensive?
The fact that multiplex PCR is the least costly diagnostic strategy for the detection of respiratory viruses was surprising and significant for a number of reasons.
How many primers are used in multiplex PCR?
Multiplex PCR requires the presence of two or more pair of primers in the reaction, so multiple genes may be amplified in one single reaction.
Is multiplex PCR quantitative?
Multiplex and real-time PCR are molecular techniques designed to amplify nucleic acid sequences in a quantitative manner.
What is triplex PCR?
Triplex real-time PCR–an improved method to detect a wide spectrum of mitochondrial DNA deletions in single cells.
How many primers are in a multiplex?
Using the SADDLE, we designed and experimentally tested multiplex primer sets comprising 192 primers (96-plex) and 784 primers (384-plex), and show low primer dimer formation through NGS experiments.
How do you make primers for multiplex PCR?
The melting temperature (Tm) of the primers should be between 58°C – 60°C, and all primers in the reaction should have a Tm within 0.5 – 1°C of each other….How to Design PCR Primers for Multiplex Assays.
| Feature | Singleplex | Multiplex |
|---|---|---|
| GC Content | 40%-60% | 40%-60% (optimal 50%) |
| Amplicon Length | 120-500 bp | 120-500 bp |