What are THP-1 cells used for?
THP-1 is a human leukemia monocytic cell line, which has been extensively used to study monocyte/macrophage functions, mechanisms, signaling pathways, and nutrient and drug transport. This cell line has become a common model to estimate modulation of monocyte and macrophage activities.
What are THP-1 derived macrophages?
THP-1 cells can be differentiated into macrophage-like cells that resemble properties of mature macrophages by activation of protein kinase C (PKC) with phorbol-12-myristate-13-acetate (PMA), ultimately resulting in cells with increased adherence and loss of proliferative activity (Schwende et al., 1996).
What is LPS stimulated?
Lipopolysaccharide (LPS) stimulates immune responses by interacting with the membrane receptor CD14 to induce the generation of cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-1, and IL-6.
Are THP-1 cells primary?
14.1 Origin and Some Features of THP-1 and U937 Cells THP-1 cells resemble primary monocytes and macrophages in morphology and differentiation properties. THP-1 cells show a large, round single-cell morphology and express distinct monocytic markers.
What does THP-1 stand for?
THP-1 is a human monocytic cell line derived from an acute monocytic leukemia patient. It is used to test leukemia cell lines in immunocytochemical analysis of protein-protein interactions, and immunohistochemistry.
How do you maintain THP-1 cells?
To Successfully Freeze THP-1 Cells, Follow These Tips:
- Thaw frozen vials in a 37°C water bath, but DO NOT LET THE WATER COME IN CONTACT WITH THE LID.
- Transfer the cells to a flask containing warmed media, then transfer some of this back into the vial to get as many cells as you can.
How do you remove a THP-1 cell?
“PBS-EDTA at 4 ° C” did not work on my THP-1…. -_-!! replace the Medium with pre warmed PBS and then add 1-2mL of 1x trypsin-EDTA and incubate at 37 for 5 minutes. All cells will be detached.
What is LPS treatment?
LPS-treatment for 6 hours increased the expression levels of pro-inflammatory and chemotactic cytokines (TNF-a, IL-1ß, IL-6, CCL2, CCL5, IL-8), whereas 48 hour-treatment elevated the expression of anti-inflammatory factors (IL-10 and IL-6). LPS led to cell injury resulting from exaggerated cell apoptosis and necrosis.
How does LPS cause inflammation?
LPS activates cells of the innate immune system, such as macrophages and neutrophils, which synthesize proinflammatory factors, such as IL-1β and TNF, MMPs and free radicals that lead to dramatic secondary inflammation in tissues.
Are THP-1 cells phagocytic?
Results. To identify genes regulating phagocytosis of the bacterium S. aureus, we selected the human monocytic cell line THP-119 as our primary screening model. THP-1 cells have been shown to spontaneously phagocytose bacteria without the need for activation or differentiation20.
Are THP-1 cells immortal?
All Answers (1) The short answer is no, the differentiated macrophages do not retain immortality. In my experience, when THP-1 cells are induced with PMA, they will stop proliferating.
Why do THP-1 cells clump?
Popular Answers (1) THP-1 cells grow in clumps as when they divide the new cell attaches to the one it split from. With my THP-1 gentle pipetting up and down will break the clumps but the cells remain viable.
What are the treatment conditions for THP-1 cells stimulated by LPS?
Treatment conditions would depend upon the question you are planning to ask using PMA differentiated THP-1 cells that are stimulated by LPS. For most purpose, a pre-treatment with PMA (10-100 nM) for 24-36 h (or longer) may be needed to fully differentiate cells. Medium can be changed following full differentiation to stimulate cells with LPS.
How do you produce IL-8 from THP-1 cells in LPs?
(A-C) IL-8 production in LPS-stimulated stably transfected THP-1 cells. Cells were added to saline (0 ng/mL LPS) or LPS (1,000, 100, 10 ng/mL) and samples placed on ice (A) or incubated for 2 h (B) or 4 h (C) at 37 °C.
Are THP-1 cells a model for human monocytes?
PMCID: PMC5124613 PMID: 27942529 THP-1 cells as a model for human monocytes Herbert Bosshart1,2and Michael Heinzelmann1,3 Herbert Bosshart 1Department of Surgery, Zurich University Hospital, CH-8091 Zurich, Switzerland; 2Private Outpatient Facility, Turnerstrasse 7a, CH-8006 Zurich, Switzerland; Find articles by Herbert Bosshart
How long does it take for THP-1 to differentiate cells?
We prime the THP-1 with 5 ng/mL PMA for 5-7 days to yield differentiated cells. They become very large and adherent, though fractions of them will die off during differentiation.